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Immunologia e infezioni

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杂交<em>原位</em>和蚊虫唾液腺，卵巢，胚胎

JoVE Journal
Immunologia e infezioni

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JoVE Journal Immunologia e infezioni

Hybridization in situ of Salivary Glands, Ovaries, and Embryos of Vector Mosquitoes

Please note that all translations are automatically generated. Click here for the English version.

杂交<em>原位</em>和蚊虫唾液腺，卵巢，胚胎

DOI:

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10:58 min

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June 28, 2012

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Jennifer Juhn, Anthony A. James²

¹Department of Molecular Biology and Biochemistry,University of California, Irvine, ²Department of Microbiology and Molecular Genetics,University of California, Irvine

Capitoli

00:05Titolo
01:25In situ Hybridization
03:27Glycerol Mounting
05:30Dechorionation, Fixation, and Endochorion Disruption
08:04Peeling
09:44Representative In situ Risultati
10:36Conclusion

Summary

Traduzione automatica

时间和空间上的基因表达分析在功能基因组学的重要作用。全挂载杂交<em>原位</em>是用于确定本地化组织和亚细胞车厢内的成绩单。在这里，我们勾勒1杂交<em>原位</em>蚊子的具体目标组织的修改协议。

Tags

Hybridization In Situ Salivary Glands Ovaries Embryos Vector Mosquitoes Pathogens Transcripts Gene Regulators Mosquito-borne Disease Transmission Egg Development Gene Expression Regulation Spatial Information Accumulation Patterns Female Salivary Glands Ovaries And Embryos Subcellular Compartments Mosquito Reproduction And Development RNA Probe Preparation Fixation And Hybridization

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