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The Neuroblast Assay: An Assay for the Generation and Enrichment of Neuronal Progenitor Cells from Differentiating Neural Stem Cell Progeny Using Flow Cytometry
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Capitoli
- 00:02Titolo
- 01:46Starting Neuroblast Assay Culture
- 04:07Switching Neuroblast Assay Medium
- 04:55Cell Preparation for Flow Cytometry
- 06:42Staining for PSA-NCAM
- 08:31Isolation of Neuronal Progenitor Cells Using Flow Cytometry
- 11:34Plating Sorted Cells
- 13:12Representative Results
- 14:34Conclusion
This video protocol demonstrates a novel method for the generation and subsequent purification of neuronal progenitor cells from a renewable source of neural stem cells (NSCs) based on their physical (size and internal granularity) and fluorescent properties using flow cytometry technology.
Tags
Neuroblast AssayGeneration Of Neuronal Progenitor CellsDifferentiating Neural Stem CellsFlow CytometryNeurosphere AssayCentral Nervous SystemAstrocytesOligodendrocytesNeuronsIn Vitro StudiesDrug ScreeningNeurotoxicologyElectrophysiologyCell Replacement TherapyHeterogeneity Of NSC ProgenyLow Production Of Neurons And OligodendrocytesPredominance Of AstrocytesClinical ApplicationsPurification Of Immature NeuronsFluorescence Activated Cell Sorting (FACS)Murine NSC ProgenyE14 Mouse Ganglionic Eminences
