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Dissection of Adult Mouse Utricle and Adenovirus-mediated Supporting-cell Infection
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JoVE Journal Neuroscienze
Dissection of Adult Mouse Utricle and Adenovirus-mediated Supporting-cell Infection

Dissection of Adult Mouse Utricle and Adenovirus-mediated Supporting-cell Infection

DOI:
10.3791/3734-v

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11:13 min

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March 28, 2012

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, , ,
1Department of Pathology and Laboratory Medicine,Medical University of South Carolina, 2Department of Microbiology & Immunology,Medical University of South Carolina, 3National Institute on Deafness and Other Communication Disorders,National Institutes of Health

Capitoli

  • 00:05Titolo
  • 02:51Utricle Dissection and Culture
  • 06:26Adenovirus Infection of Supporting Cells
  • 07:39Utricle Fixation, Otoconia Removal, and Immunochemistry
  • 09:06Results: Adenovirus-mediated Infection of Supporting Cells
  • 10:19Conclusion

Summary

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Mechanosensory hair cells are the receptor cells of the inner ear. The best-characterized in vitro model system of mature mammalian hair cells utilizes organ cultures of utricles from adult mice. We present the dissection of the adult mouse utricle, and we demonstrate adenovirus-mediated infection of supporting cells in cultured utricles.

Tags

DissectionAdult Mouse UtricleAdenovirus-mediated InfectionHair CellsInner EarPrimary Organ CulturesUtriclesVestibular OrganOrgan Of CortiMolecular SignalsSurvivalHomeostasisDeathCochlear Hair CellsRegenerationGlia-like Supporting CellsBasolateral SurfacesSensory EpitheliumAdenovirus Type 5 (dE1/E3)

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