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Capitoli
- 00:05Titolo
- 01:08Preparation of Bacterial Inocula and Agar Plates for Enumeration of Inocula
- 02:23Collection of Squid Juveniles
- 04:18Squid Colonization
- 06:33Determination of Colonization Levels
- 07:45Representative Colonization Results
- 08:31Conclusion
The method outlines the procedure by which the Hawaiian bobtail squid, Euprymna scolopes and its bacterial symbiont, Vibrio fischeri, are raised separately and then introduced to allow for specific colonization of the squid light organ by the bacteria. Colonization detection by bacterially-derived luminescence and by direct colony counting are described.
