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Capitoli
- 00:05Titolo
- 01:20Culturing Human Embryonic Stem Cells
- 02:05Definitive Endoderm and Pancreatic Progenitor Induction
- 03:01Pancreatic Maturation
- 03:57qRT-PCR Analysis
- 05:06Immunocytochemistry
- 06:21Analysis of Differentiation of Human Embryonic Stem Cells into Insulin-producing Cells
- 08:07Conclusion
The current study describes a directed differentiation approach in inducing pancreatic differentiation of human embryonic stem cells. Of great significance is the finding that endothelial cell co-culture mediates maturation of human embryonic stem cell derived pancreatic progenitors into insulin expressing cells.
Tags
Endothelial Cell Co-cultureHuman Embryonic Stem CellsDirected Differentiation ApproachPancreatic Insulin Producing CellsEmbryonic Stem CellsPluripotentCell Based TherapyRegenerative TreatmentCellular DifferentiationOrganogenesisPancreas DevelopmentEndoderm InductionNodal Pathway ModulationActivin AGrowth FactorsSonic Hedgehog InhibitionCyclopaminePancreatic MaturationNotch Signaling InhibitionVascularization
