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Endotel Cell Co-kultur formidler Modning af humane embryonale stamceller til pancreas-insulin-producerende celler i en rettet Differentiering Approach
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Biologia
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JoVE Journal Biologia
Endothelial Cell Co-culture Mediates Maturation of Human Embryonic Stem Cell to Pancreatic Insulin Producing Cells in a Directed Differentiation Approach

Endotel Cell Co-kultur formidler Modning af humane embryonale stamceller til pancreas-insulin-producerende celler i en rettet Differentiering Approach

DOI:
10.3791/3759-v

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08:29 min

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March 27, 2012

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,
1Department of Bioengineering,University of Pittsburgh, 2Department of Chemical Engineering,University of Pittsburgh

Capitoli

  • 00:05Titolo
  • 01:20Culturing Human Embryonic Stem Cells
  • 02:05Definitive Endoderm and Pancreatic Progenitor Induction
  • 03:01Pancreatic Maturation
  • 03:57qRT-PCR Analysis
  • 05:06Immunocytochemistry
  • 06:21Analysis of Differentiation of Human Embryonic Stem Cells into Insulin-producing Cells
  • 08:07Conclusion

Summary

Traduzione automatica

Traduzione automatica

Den aktuelle undersøgelse beskriver en rettet differentiering tilgang til at fremkalde bugspytkirtlen differentiering af humane embryonale stamceller. Af stor betydning er konstateringen af, at endotelcelle co-kultur medierer modning af humane embryonale stamceller afledt pancreas stamfædre til insulin udtrykker celler.

Tags

Endothelial Cell Co-cultureHuman Embryonic Stem CellsDirected Differentiation ApproachPancreatic Insulin Producing CellsEmbryonic Stem CellsPluripotentCell Based TherapyRegenerative TreatmentCellular DifferentiationOrganogenesisPancreas DevelopmentEndoderm InductionNodal Pathway ModulationActivin AGrowth FactorsSonic Hedgehog InhibitionCyclopaminePancreatic MaturationNotch Signaling InhibitionVascularization

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