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Endotelcelle Co-kulturen formidler Modning av Human embryonale stamceller til Bukspyttkjertelen insulinproduserende celler i en Regissert Differensiering Approach
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Biologia
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JoVE Journal Biologia
Endothelial Cell Co-culture Mediates Maturation of Human Embryonic Stem Cell to Pancreatic Insulin Producing Cells in a Directed Differentiation Approach

Endotelcelle Co-kulturen formidler Modning av Human embryonale stamceller til Bukspyttkjertelen insulinproduserende celler i en Regissert Differensiering Approach

DOI:
10.3791/3759-v

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08:29 min

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March 27, 2012

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,
1Department of Bioengineering,University of Pittsburgh, 2Department of Chemical Engineering,University of Pittsburgh

Capitoli

  • 00:05Titolo
  • 01:20Culturing Human Embryonic Stem Cells
  • 02:05Definitive Endoderm and Pancreatic Progenitor Induction
  • 03:01Pancreatic Maturation
  • 03:57qRT-PCR Analysis
  • 05:06Immunocytochemistry
  • 06:21Analysis of Differentiation of Human Embryonic Stem Cells into Insulin-producing Cells
  • 08:07Conclusion

Summary

Traduzione automatica

Traduzione automatica

Den nåværende studien beskriver en rettet differensiering tilnærming i inducing bukspyttkjertelen differensiering av humane embryonale stamceller. Av stor betydning er det faktum at endotelceller co-kulturen formidler modning av humane embryonale stamceller hentet bukspyttkjertelen stamfedre til insulin som uttrykker cellene.

Tags

Endothelial Cell Co-cultureHuman Embryonic Stem CellsDirected Differentiation ApproachPancreatic Insulin Producing CellsEmbryonic Stem CellsPluripotentCell Based TherapyRegenerative TreatmentCellular DifferentiationOrganogenesisPancreas DevelopmentEndoderm InductionNodal Pathway ModulationActivin AGrowth FactorsSonic Hedgehog InhibitionCyclopaminePancreatic MaturationNotch Signaling InhibitionVascularization

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