Rivista
/
Immunologia e infezioni
/
TransFLP - A基因修改方法<em>霍乱弧菌</em基于自然转化和FLP重组
JoVE Journal
Immunologia e infezioni
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Immunologia e infezioni
TransFLP — A Method to Genetically Modify Vibrio cholerae Based on Natural Transformation and FLP-recombination
Please note that all translations are automatically generated. Click here for the English version.

TransFLP - A基因修改方法<em>霍乱弧菌</em基于自然转化和FLP重组

DOI:
10.3791/3761-v

•

12:13 min

•

October 08, 2012

•

1Global Health Institute, School of Life Sciences,Ecole Polytechnique Fédérale de Lausanne (EPFL)

Capitoli

  • 00:05Titolo
  • 01:16Preparation of Chitin Flakes
  • 01:39Oligonucleotide Design and Polymerase Chain Reaction
  • 03:45Chitin-induced Natural Transformation
  • 04:59Removal of Selective Cassette(s) by FLP-recombination
  • 05:46Plasmid Curing
  • 06:21Results: Representative TransFLP Modifications of V. cholerae
  • 11:34Conclusion

Summary

Traduzione automatica

Traduzione automatica

一个快速的方法来修改的基因组<em> V.霍乱</em>进行说明。这些修改包括删除单个基因，基因簇和基因组岛屿以及集成的短序列（如启动子元件或亲和标签序列）。该方法是基于对自然的改造和FLP重组。

Tags

TransFLPGenetically ModifyVibrio CholeraeNatural TransformationFLP-recombinationBacterial ChromosomesReplicative PlasmidsHomology-mediated RecombinationInsertional MutantsPlasmid ExcisionGram-negative BacteriaLevan Sucrase EnzymeSacB GenePre-insertion GenotypeModified GeneTime-consumingCloningHorizontal TransferArtificial TransformationPositive SelectionRapid ManipulationChitin-mediated InductionNatural Competence

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

定植 Euprymna scolopes鱿鱼由弧菌鲵</em

Antileishmanial筛选对细胞内的寄生虫救援和转化实验<em>杜氏利什曼原虫</em无鞭毛体在THP1人急性单核细胞白血病细胞株

临床应用<em>睡美人</em和人工抗原呈递细胞基因修饰T细胞从外设和脐带血中

的自然杀伤细胞免疫突触上的玻璃支承平面脂双层超分辨率成像

基于插入渗透膜感染系统的实现研究分泌细菌病毒对哺乳动物宿主细胞的作用

对于检测到的敏感性和特异性显色琼脂培养基发展<em>副溶血性弧菌</em>及其他<em>弧菌</em>物种

适合与天然化合物库的探索抗生物试验的平台

荧光为基础的神经氨酸酶抑制测定流感病毒的易感性评估抗病毒药的神经氨酸酶抑制剂类

用于维持和区分生物型的实验室技术<em>霍乱弧菌</em>临床和环境隔离

一种新型大鼠自然杀伤细胞无饲养系统的研究体外

Read Article