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龙虎斗动粒蛋白的动态行为与染色体运动，在生活学习有丝分裂关卡<em>果蝇</em>合胞胚胎
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Studying Mitotic Checkpoint by Illustrating Dynamic Kinetochore Protein Behavior and Chromosome Motion in Living Drosophila Syncytial Embryos
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龙虎斗动粒蛋白的动态行为与染色体运动，在生活学习有丝分裂关卡<em>果蝇</em>合胞胚胎

DOI:
10.3791/3763-v

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13:59 min

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June 14, 2012

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,
1Institute for Cell and Molecular Biosciences,University of Newcastle, United Kingdom

Capitoli

  • 00:05Titolo
  • 01:45Maintaining Flies and Collecting Eggs
  • 03:43Preparing Coverslips and Slides
  • 05:10Dechorionating Embryos
  • 06:36Provoking the SAC by Microinjecting Embryos with Appropriate Reagents
  • 08:55Imaging Embryos
  • 10:08Results: Observing Dynamic Kinetochore Behavior and Chromosome Motion
  • 13:28Conclusion

Summary

Traduzione automatica

Traduzione automatica

着丝粒是地方国资委启动其信号监测的有丝分裂的姐妹染色单体隔离。一种方法是描述与染色体运动的可视化招聘和营业额的着丝粒蛋白及其协调<em>果蝇</em>胚胎使用徕卡激光扫描共聚焦系统。

Tags

Mitotic CheckpointKinetochore ProteinChromosome MotionLiving Drosophila Syncytial EmbryosSAC MechanismAneuploidyGenomic InstabilityCancersBirth DefectsAlzheimer’sMad1Mad2Bub1BubR1Bub3Mps1Zw10RodAurora B KinaseCdc20APC/C ActivatorsCyclin BSecurinMetaphase To Anaphase TransitionSAC Signal InitiationKinetochore AssemblyRelay Onto APC/C InhibitionDrosophila Experimental System

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