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Capitoli
- 00:05Titolo
- 01:45Maintaining Flies and Collecting Eggs
- 03:43Preparing Coverslips and Slides
- 05:10Dechorionating Embryos
- 06:36Provoking the SAC by Microinjecting Embryos with Appropriate Reagents
- 08:55Imaging Embryos
- 10:08Results: Observing Dynamic Kinetochore Behavior and Chromosome Motion
- 13:28Conclusion
SAC kardeş kromatidler arasında mitotik segregasyon izlenmesi, sinyali başlatır nerede kinetochore olduğunu. A metodunda kromozom hareketi ile kinetochore proteinler ve koordinasyon birinin seçilmesi ve devir görselleştirme tarif edilmektedir<em> Drosophila</em> Leica lazer tarama konfokal sistemi kullanılarak embriyo.
Tags
Mitotic CheckpointKinetochore ProteinChromosome MotionLiving Drosophila Syncytial EmbryosSAC MechanismAneuploidyGenomic InstabilityCancersBirth DefectsAlzheimer’sMad1Mad2Bub1BubR1Bub3Mps1Zw10RodAurora B KinaseCdc20APC/C ActivatorsCyclin BSecurinMetaphase To Anaphase TransitionSAC Signal InitiationKinetochore AssemblyRelay Onto APC/C InhibitionDrosophila Experimental System
