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Profilering Thiol Redox Proteome Brug Isotope Tagging massespektrometri
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Biologia
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JoVE Journal Biologia
Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry

Profilering Thiol Redox Proteome Brug Isotope Tagging massespektrometri

DOI:
10.3791/3766-v

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12:07 min

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March 24, 2012

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, , ,
1Plant Molecular and Cellular Biology Program,University of Florida , 2Department of Biology,University of Florida , 3Interdisciplinary Center for Biotechnology Research,University of Florida , 4Genetics Institute,University of Florida

Capitoli

  • 00:05Titolo
  • 02:07Inoculation of Tomato
  • 03:13Protein Extraction
  • 04:21Sample Preparation and Peptide Labeling with cysTMT
  • 06:07Removal of Non-reacted Tag Sample Fractionation
  • 07:44Sample Enrichment of cysTMT-labeled Peptides
  • 08:43Mass Spectrometry Analysis
  • 10:02CysTMT Results: Profiling Thiol Redox Proteome of Pseudomonas syringae in Tomato
  • 11:11Conclusion

Summary

Traduzione automatica

Traduzione automatica

Reaktive oxygenformer niveau hæves, når celler støder stressbetingelser. Her viser vi eksempel 3'-3 'diaminobenzidin farvning såvel som cysTMT mærkning og massespektrometri for at profilere redox proteom med<em> Pseudomonas syringae</em> Behandlet tomatblade.

Tags

Thiol Redox ProteomeIsotope Tagging Mass SpectrometryPseudomonas Syringae Pv. TomatoBacterial Speck DiseaseSolanum LycopersicumBrassica SpeciesArabidopsis ThalianaReactive Oxygen Species (ROS)Hydrogen PeroxideDAB StainingRedox Status Of ProteinsCysteine OxidationTandem Mass Tag (TMT) ReagentsCysteine-reactive TMT (cysTMT) Tags

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