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Profilering Thiol Redox Proteome Met behulp van Isotope Tagging massaspectrometrie
JoVE Journal
Biologia
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JoVE Journal Biologia
Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry
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Profilering Thiol Redox Proteome Met behulp van Isotope Tagging massaspectrometrie

DOI:
10.3791/3766-v

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12:07 min

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March 24, 2012

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, , ,
1Plant Molecular and Cellular Biology Program,University of Florida , 2Department of Biology,University of Florida , 3Interdisciplinary Center for Biotechnology Research,University of Florida , 4Genetics Institute,University of Florida

Capitoli

  • 00:05Titolo
  • 02:07Inoculation of Tomato
  • 03:13Protein Extraction
  • 04:21Sample Preparation and Peptide Labeling with cysTMT
  • 06:07Removal of Non-reacted Tag Sample Fractionation
  • 07:44Sample Enrichment of cysTMT-labeled Peptides
  • 08:43Mass Spectrometry Analysis
  • 10:02CysTMT Results: Profiling Thiol Redox Proteome of Pseudomonas syringae in Tomato
  • 11:11Conclusion

Summary

Traduzione automatica

Traduzione automatica

Reactive oxygen species niveau wordt verhoogd wanneer de cellen tegenkomen stress. Hier laten we het voorbeeld van 3'-3 'diaminobenzidine vlekken en cysTMT etikettering en massaspectrometrie om de redox proteoom profileren in<em> Pseudomonas syringae</em> Behandeld tomaat bladeren.

Tags

Thiol Redox ProteomeIsotope Tagging Mass SpectrometryPseudomonas Syringae Pv. TomatoBacterial Speck DiseaseSolanum LycopersicumBrassica SpeciesArabidopsis ThalianaReactive Oxygen Species (ROS)Hydrogen PeroxideDAB StainingRedox Status Of ProteinsCysteine OxidationTandem Mass Tag (TMT) ReagentsCysteine-reactive TMT (cysTMT) Tags

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