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衍生Aldononitrile乙酸氨基葡萄糖和胞壁酸的微生物在土壤中残留量的测定气相色谱的检测

JoVE Journal
Biologia

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JoVE Journal Biologia

GC-based Detection of Aldononitrile Acetate Derivatized Glucosamine and Muramic Acid for Microbial Residue Determination in Soil

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衍生Aldononitrile乙酸氨基葡萄糖和胞壁酸的微生物在土壤中残留量的测定气相色谱的检测

DOI:

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11:06 min

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May 19, 2012

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Chao Liang², Harry W. Read, Teri C. Balser³

¹DOE-Great Lakes Bioenergy Research Center,University of Wisconsin, Madison, ²Department of Soil Science,University of Wisconsin, Madison, ³Department of Soil and Water Science,University of Florida

Capitoli

00:05Titolo
01:52Sample Preparation and Purification
05:06Derivatization
06:06Separation and Measurement
08:11Representative Results
09:55Conclusion

Summary

Traduzione automatica

我们描述了一个协议aldonitrile从土壤中提取的葡萄糖和胞壁酸醋酸衍生物GC为基础的分析方法。对于澄清的化学机制，我们也提出一项战略，以确认衍生工具及电子电离后形成的碎片离子的结构。

Tags

GC-based Detection Aldononitrile Acetate Derivatized Glucosamine Muramic Acid Microbial Residue Determination Soil Microbial Analysis Biomarker Analysis Glucosamine And Muramic Acid Analysis Analytical Methods Derivatization GC-based Analysis Precision Sensitivity Chromatographic Separation Sample Storage

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