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Capitoli
- 00:05Titolo
- 01:05Conjugation of Annexin V to SPIO and Dynamic Light Scattering
- 03:19Cell Culture, Induction of Apoptosis in Culture, and MRI of Cultured Cells
- 04:16In vitro MRI
- 05:32Representative MRI Results
- 06:38Conclusion
Early detection of apoptosis may identify at-risk cell populations in a variety of diseases. Here we demonstrate a method to link an early apoptosis-detection protein (Annexin V) to a MRI-detectable iron oxide nanoparticle (SPIO). This method may be extended to other proteins of interest to generate MRI-detectable molecular imaging probes.
Tags
In Vivo MRI-detectable Apoptosis ProbeCellular ApoptosisDiseasesDetect ApoptosisReversible StagesPre-clinical WindowPreventive MeasuresTherapeutic MeasuresProtect The HeartPermanent DamageAnnexin V (ANX)Superparamagnetic Iron Oxide (SPIO) NanoparticlesConjugation MethodOxidationCarboxyl GroupsPolysaccharide ShellMRI-detectable Contrast AgentReducing BufferSodium BorohydrideReaction QuenchingMicrocentrifuge FiltrationDynamic Light Scattering (DLS)Cross-linked Iron Oxide Particle Conjugation MethodsBiotin-labeled Nanoparticles
