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Skin Punch Biopsy Explant Culture for Derivation of Primary Human Fibroblasts
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JoVE Journal Medicina
Skin Punch Biopsy Explant Culture for Derivation of Primary Human Fibroblasts

Skin Punch Biopsy Explant Culture for Derivation of Primary Human Fibroblasts

DOI:
10.3791/3779-v

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06:19 min

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July 07, 2013

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, , , ,
1Basic Research Department,The Parkinson’s Institute

Capitoli

  • 00:01Titolo
  • 00:11Required Materials
  • 01:09Step 1. Transferring biopsy from tube to cutting dish
  • 02:16Step 2. Dissection of the skin biopsy
  • 03:28Step 3. Transfer biopsy pieces to culture plate
  • 05:19Representative Results
  • 06:38Conclusion

Summary

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The fibroblast explant culture protocol from human skin punch biopsies is a technically robust and simple way to derive skin cells within 4-8 weeks for banking of about 15-20 million cells at a low passage number.

Tags

Skin Punch BiopsyExplant CulturePrimary Human FibroblastsDisease StudyInduced Pluripotent Stem CellsCell-based Functional AssaysDisease PathwaysDrug ScreeningParkinson’s DiseaseEnzymatic ProceduresReproducibilityTechnical SimplicityTime ConsiderationCell ViabilityAttachment Of CellsDissectionPreparationKeratinocytes

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