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In Ovo Electroporation in Embryonic Chick Retina
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Biologia
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JoVE Journal Biologia
In Ovo Electroporation in Embryonic Chick Retina

In Ovo Electroporation in Embryonic Chick Retina

DOI:
10.3791/3792-v

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06:15 min

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February 05, 2012

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, ,
1Department of Pharmacology,University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, 2Department of Biomedical Engineering,Rutgers University

Capitoli

  • 00:05Titolo
  • 00:56Egg Handling and Needle Preparation
  • 03:20Injection and Electroporation

Summary

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The overall goal of this video is to show how to perform targeted retinal injection and in ovo electroporation of DNA/RNA constructs into the chick embryonic retina at the Hamburger and Hamilton stage 22-23, which is about embryonic day 4 (E4). This technique is very useful to study gene expression, gene regulation, and morphological change in developing chick retina.

Tags

Ovo ElectroporationEmbryonic Chick RetinaRetinal DevelopmentGene RegulationRecombinant DNA/RNA TechnologyReporter Gene AssayGene Over-expressionGene Knock DownTargeted Retinal InjectionHamburger And Hamilton Stage 22-23Embryonic Day 4 (E4)Green Fluorescent Protein (GFP)Subretinal SpaceRetinal Stem/progenitor CellsLate-born Neurons

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