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荧光显微镜筛选和新一代测序:参与细胞器完整性的基因鉴定的有用工具
JoVE Journal
Biologia
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JoVE Journal Biologia
Fluorescence-microscopy Screening and Next-generation Sequencing: Useful Tools for the Identification of Genes Involved in Organelle Integrity
DOI:

12:42 min

April 13, 2012

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Capitoli

  • 00:05Titolo
  • 01:20Ethyl Methane Sulfonate (EMS) Treatment
  • 04:25Confocal Screening of M2 and M3 Populations
  • 05:35Mapping
  • 09:10Results: Mutation Mapping using Genechip Arabidopsis ATH1 Genome Array Hybridization and R Software Analysis
  • 12:24Conclusion

Summary

Traduzione automatica

在细胞生物学的一个基本任务是定义机制的基础,使真核细胞的细胞器的身份。在这里,我们提出了一个方法,以确定该基因的植物细胞器,利用荧光显微镜和下一代测序工具的形态和功能的完整性负责。

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