È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:05Titolo
- 02:05Dissection of Neonatal Brain Tissue
- 03:28Preparation of Mixed Glial Cell Population
- 04:36Plating and Maintenance of Mixed Glial Cell Cultures
- 05:36Isolation and Plating of Primary Microglia
- 06:50Results: Cell-type Specific Immunostaining and Response of Microglial Cultures to Lipopolysaccharide
- 08:46Conclusion
Tags
Primary Microglia IsolationMixed Glial Cell CulturesMicroglia Role In Brain PhysiologyPhagocytic ActivityMicroglial ActivationNeurodegenerative DiseaseTraumatic Brain InjuryNervous System InfectionReactive Oxygen And Nitrogen SpeciesPro-inflammatory Chemokines And CytokinesCellular Heterogeneity Of The BrainNeuroprotective And Neurotoxic Functions Of MicrogliaIsolation Protocol For Primary Microglia
