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Measurement of Cellular Chemotaxis with ECIS/Taxis
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Biologia
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JoVE Journal Biologia
Measurement of Cellular Chemotaxis with ECIS/Taxis

Measurement of Cellular Chemotaxis with ECIS/Taxis

DOI:
10.3791/3840-v

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11:37 min

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April 01, 2012

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, , ,
1Molecular and Cell Biology,University of Connecticut, 2University of Connecticut

Capitoli

  • 00:05Titolo
  • 01:28ECIS/Taxis Electrode Preparation
  • 03:13Preparing Agarose Chambers and the Cannula Well-cutting Tool
  • 04:21Cutting Wells into Agarose
  • 05:51Loading the Wells
  • 07:04Data Collection
  • 08:55Data Management
  • 10:13Results: Chemotaxis of Human Jurkat T cells in Response to SDF1alpha
  • 11:14Conclusion

Summary

Traduzione automatica

Traduzione automatica

The ECIS/Taxis system is an automated, real-time assay that measures cellular chemotaxis. In this assay, cells move beneath a layer of agarose to arrive at a target electrode. Cellular movement is measured by the onset of resistance to AC current 0.

Tags

Cellular ChemotaxisECISTaxisCellularBoyden Chamber AssayUnder-agarose Chemotaxis AssayChemoattractant GradientSolid SubstrateAqueous FilmAgarose LayerAutomated ECIS/Taxis SystemElectric Cell-substrate Impedance SensingTarget ElectrodesCounter-electrode

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