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Måling af cellulære Kemotaksi med ECIS / Taxa
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Biologia
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JoVE Journal Biologia
Measurement of Cellular Chemotaxis with ECIS/Taxis

Måling af cellulære Kemotaksi med ECIS / Taxa

DOI:
10.3791/3840-v

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11:37 min

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April 01, 2012

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, , ,
1Molecular and Cell Biology,University of Connecticut, 2University of Connecticut

Capitoli

  • 00:05Titolo
  • 01:28ECIS/Taxis Electrode Preparation
  • 03:13Preparing Agarose Chambers and the Cannula Well-cutting Tool
  • 04:21Cutting Wells into Agarose
  • 05:51Loading the Wells
  • 07:04Data Collection
  • 08:55Data Management
  • 10:13Results: Chemotaxis of Human Jurkat T cells in Response to SDF1alpha
  • 11:14Conclusion

Summary

Traduzione automatica

Traduzione automatica

Den ECIS / Taxa system er en automatiseret, real-time analyse, der måler cellulære kemotaksi. I dette assay flytte cellerne under et lag af agarose at nå frem til et mål elektrode. Cellulær bevægelse måles ved indtræden af ​​resistens over for vekselstrøm 0.

Tags

Cellular ChemotaxisECISTaxisCellularBoyden Chamber AssayUnder-agarose Chemotaxis AssayChemoattractant GradientSolid SubstrateAqueous FilmAgarose LayerAutomated ECIS/Taxis SystemElectric Cell-substrate Impedance SensingTarget ElectrodesCounter-electrode

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