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囊性纤维化跨膜传导调节蛋白表达和纯化酿酒酵母
JoVE Journal
Biologia
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JoVE Journal Biologia
Expression and Purification of the Cystic Fibrosis Transmembrane Conductance Regulator Protein in Saccharomyces cerevisiae
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囊性纤维化跨膜传导调节蛋白表达和纯化酿酒酵母

DOI:
10.3791/3860-v

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14:56 min

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March 10, 2012

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, , ,
1Faculty of Life Sciences,University of Manchester

Capitoli

  • 00:05Titolo
  • 03:42Screening Transformants
  • 07:35Large-scale Fermenter Culture
  • 11:37Representative Yeast Transformation Results
  • 13:27Conclusion

Summary

Traduzione automatica

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企图表达的囊性纤维化跨膜电导调节器（CFTR）<em>酿酒酵母</em>，到现在为止，已产生的蛋白质含量相对较低。通过囊性纤维化基金会分发本协议及相关的试剂应允许毫克这个'困难'真核生物膜蛋白的准备。

Tags

Cystic Fibrosis Transmembrane Conductance RegulatorCFTR ProteinExpressionPurificationSaccharomyces CerevisiaeChloride ChannelCystic FibrosisProtein StructureProtein ActivityRecombinant ProteinYeast S. CerevisiaeProteolysis ProblemsCell Growth ConditionsInduction ConditionsReagentsUS Cystic Fibrosis Foundation

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