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Ekspression og oprensning af cystisk fibrose-transmembrankonduktans-regulator protein i Saccharomyces cerevisiae
JoVE Journal
Biologia
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JoVE Journal Biologia
Expression and Purification of the Cystic Fibrosis Transmembrane Conductance Regulator Protein in Saccharomyces cerevisiae

Ekspression og oprensning af cystisk fibrose-transmembrankonduktans-regulator protein i Saccharomyces cerevisiae

DOI:
10.3791/3860-v

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14:56 min

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March 10, 2012

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, , ,
1Faculty of Life Sciences,University of Manchester

Capitoli

  • 00:05Titolo
  • 03:42Screening Transformants
  • 07:35Large-scale Fermenter Culture
  • 11:37Representative Yeast Transformation Results
  • 13:27Conclusion

Summary

Traduzione automatica

Traduzione automatica

Forsøg på at udtrykke den cystisk fibrose transmembran konduktans regulator (CFTR) i<em> Saccharomyces cerevisiae</em> Har indtil nu gav relativt lave mængder af protein. Denne protokol og de tilhørende reagenser distribueres via cystisk fibrose Foundation bør tillade udarbejdelse af milligram mængder af denne 'svære' eukaryot membranprotein.

Tags

Cystic Fibrosis Transmembrane Conductance RegulatorCFTR ProteinExpressionPurificationSaccharomyces CerevisiaeChloride ChannelCystic FibrosisProtein StructureProtein ActivityRecombinant ProteinYeast S. CerevisiaeProteolysis ProblemsCell Growth ConditionsInduction ConditionsReagentsUS Cystic Fibrosis Foundation

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