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MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR (qPCR)
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JoVE Journal Medicina
MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR (qPCR)

MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR (qPCR)

DOI:
10.3791/3874-v

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08:30 min

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May 16, 2012

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, , , ,
1Department of Laboratory Medicine & Pathobiology,University of Toronto, 2Division of Urology,Sunnybrook Health Sciences Centre, Toronto, Canada, 3Department of Anatomic Pathology,Sunnybrook Health Sciences Centre, Toronto, Canada, 4Biological Sciences,Sunnybrook Research Institute

Capitoli

  • 00:05Titolo
  • 01:51Prostate Sample Collection
  • 02:40Isolating Total RNA from Samples
  • 04:27Real-time PCR for Detection of miRNA
  • 06:10Analyzing the Data
  • 07:25Representative qPCR Results
  • 08:02Conclusion

Summary

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Quantitative Real Time polymerase chain reaction (qPCR) is a rapid and sensitive method to investigate the expression levels of various microRNA (miRNA) molecules in tumor samples. Using this method expression of hundreds of different miRNA molecules can be amplified, quantified, and analyzed from the same cDNA template.

Tags

MicroRNA DetectionProstate TumorsQuantitative Real-time PCR (qPCR)MiRNAsNon-coding RNA MoleculesCellular ProcessDevelopmentApoptosisCell Cycle RegulationGene Expression RegulationTarget Messenger RNAs (mRNAs)DysregulationBiomarkersTherapeutic AgentsTaqman Probe-based QPCRClinical SamplesAccuracyReproducibilitySpecificityWhole Genome AnalysesMicroarray StudiesRadical ProstatectomyClinical Variables

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