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Capitoli
- 00:05Titolo
- 03:11Generation of a Single-cell Suspension of Leukocytes from Spleens
- 05:09Cell Surface Staining/Immunophenotyping of MDSC using Flow Cytometry
- 09:01Magnetic Enrichment of Gr-1+ Leukocytes
- 12:06Post-sort Analysis of Gr-1-enriched Leukocytes
- 12:51AutoMACS Enrichment of Naive Gr-1+ Leukocytes for MDSC FACS Sorting
- 13:40Conclusion
Este es un método rápido y completo de inmunofenotipo mieloide derivados de células supresoras (MDSC) y enriquecedora Gr-1<sup> +</sup> Leucocitos de bazos de ratones. Este método utiliza la citometría de flujo y Celular AutoMACS clasificación para enriquecer viables Gr-1<sup> +</sup> Leucocitos antes de FACS clasificación de MDSC para su uso<em> En vivo</em> Y<em> In vitro</em> Ensayos.
Tags
Myeloid Derived Suppressor Cells (MDSC)Naive MicePancreatic Tumor-bearing MiceFlow CytometryAutomated Magnetic Activated Cell Sorting (AutoMACS)Heterogeneous PopulationImmature MacrophagesDendritic CellsGranulocytesLymphoid OrgansParasitic InfectionInflammationTraumatic StressGraft-versus-host DiseaseDiabetesCancerMac-1 (CD11b)Gr-1 (Ly6G And Ly6C)Tumor-bearing HostsAnti-tumor Immune ResponsesSubpopulations Of MDSCMolecular Mechanisms Of SuppressionGhansah GroupMurine Pancreatic Cancer ModelHomeostasisSuppressive FunctionLymphoid CompartmentsGr-1+ Leukocytes
