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In vitro Mesothelial Clearance Assay that Models the Early Steps of Ovarian Cancer Metastasis
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JoVE Journal Medicina
In vitro Mesothelial Clearance Assay that Models the Early Steps of Ovarian Cancer Metastasis

In vitro Mesothelial Clearance Assay that Models the Early Steps of Ovarian Cancer Metastasis

DOI:
10.3791/3888-v

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08:54 min

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February 17, 2012

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, ,
1Department of Cell Biology,Harvard Medical School

Capitoli

  • 00:05Titolo
  • 01:22Ovarian Cancer Cell Spheroid Formation
  • 03:28Mesothelial Cell Monolayer Formation
  • 06:15Results: Analysis of Mesothelial Clearance by Ovarian Cancer Cell Spheroids
  • 08:32Conclusion

Summary

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The mesothelial clearance assay described here takes advantage of fluorescently labeled cells and time-lapse video microscopy to visualize and quantitatively measure the interactions of ovarian cancer multicellular spheroids and mesothelial cell monolayers. This assay models the early steps of ovarian cancer metastasis.

Tags

In Vitro Mesothelial Clearance AssayOvarian Cancer MetastasisSecondary MetastasesPeritoneal CavityMulticellular ClustersSpheroidsMesothelial CellsPeritoneal Tumor MassesExtracellular MatrixPrimary Ovarian Cancer CellsPrimary Peritoneal Mesothelial Cells

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