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Purification and Visualization of Lipopolysaccharide from Gram-negative Bacteria by Hot Aqueous-phenol Extraction
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Purification and Visualization of Lipopolysaccharide from Gram-negative Bacteria by Hot Aqueous-phenol Extraction

Purification and Visualization of Lipopolysaccharide from Gram-negative Bacteria by Hot Aqueous-phenol Extraction

DOI:
10.3791/3916-v

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05:31 min

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May 28, 2012

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1Department of Microbiology, Immunology, & Cancer Biology,University of Virginia Health System

Capitoli

  • 00:05Titolo
  • 01:38Preparation of Bacteria and LPS Extraction
  • 04:21LPS Purification Results
  • 04:48Conclusion

Summary

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We describe a modified hot aqueous-phenol extraction method for purifying lipopolysaccharide (LPS) from Gram-negative bacteria. Once extracted, the LPS can be subsequently analyzed by SDS-PAGE and visualized by direct staining or Western immunoblot.

Tags

PurificationVisualizationLipopolysaccharideGram-negative BacteriaHot Aqueous-phenol ExtractionLipid ACore OligosaccharideO-antigen UnitsVirulencePathogenesisSerotypingClinical IsolatesAntibodiesMutantsExtraction MethodNucleic AcidsProteinsSodium Dodecy

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