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Hydrophobic Salt-modified Nafion for Enzyme Immobilization and Stabilization
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Bioengineering
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JoVE Journal Bioengineering
Hydrophobic Salt-modified Nafion for Enzyme Immobilization and Stabilization

Hydrophobic Salt-modified Nafion for Enzyme Immobilization and Stabilization

DOI:
10.3791/3949-v

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11:16 min

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July 11, 2012

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, , ,
1Departments of Chemistry and Materials Science and Engineering,University of Utah

Chapters

  • 00:05Title
  • 01:47Modification of Nafion with Quaternary Ammonium Salts
  • 04:55Immobilization of Enzymes into TBAB-modified Nafion for Activity Assays
  • 05:56Assay of Immobilized NAD-dependent Dehydrogenase
  • 07:05Assay of Immobilized PQQ-dependent Dehydrogenases
  • 08:15Assay of Immobilized Glucose Oxidase
  • 09:11Results: Activity of Enzymes Immobilized on Various Modified Nafion Films
  • 11:00Conclusion

Summary

Automatic Translation

Automatic Translation

This article will describe the procedure for synthesizing a hydrophobically modified Nafion enzyme immobilization membrane and how to immobilize proteins and/or enzymes within the membrane and test their specific activity.

Tags

Hydrophobic Salt-modified NafionEnzyme ImmobilizationEnzyme StabilizationBiocatalysisBiosensorsBiofuel CellsPolymer MatrixPolyaminesPolyalcoholsMicellesMinteer GroupNafionAlkyl Ammonium SaltsTetrabutylammonium Bromide (TBAB)

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