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Endothelialisierten Mikrofluidik für ein Studium Mikrovaskuläre Wechselwirkungen in Hämatologische Krankheiten
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1Department of Pediatrics,Emory University School of Medicine, 2Wallace H. Coulter Department of Biomedical Engineering,Georgia Institute of Technology and Emory University, 3Aflac Cancer Center and Blood Disorders Service of Children’s Healthcare of Atlanta , 4Winship Cancer Institute of Emory University
Capitoli
- 00:05Titolo
- 01:11Fabrication of the Endothelial Microdevice
- 03:46Polydimethylsiloxane (PDMS) Preparation
- 06:25Seeding the Microfluidic Device with Endothelial Cells
- 08:49Endothelialized Microfluidics Reveal Abnormal Microvascular Interactions after Infusion with Blood from Patients with Hematologic Disease
- 10:35Conclusion
Verfahren zur Kultur von Endothelzellen eine Monoschicht der gesamten inneren Oberfläche des 3D einer Mikrofluidikvorrichtung mit mikrovaskulären großen Kanälen (<30 um) beschrieben. Dies<em> In-vitro-</em> Mikrovaskulatur Modell ermöglicht die Untersuchung der biophysikalischen Wechselwirkungen zwischen Blutzellen, Endothelzellen, und löslichen Faktoren in hämatologischen Erkrankungen.
Tags
MicrofluidicsEndothelialized MicrofluidicsMicrovascular InteractionsHematologic DiseasesMicrofabrication TechniquesBiological ExperimentsBiochemical ExperimentsMicro- And NanoscalesHematologic ProcessesBlood Cell DeformabilityBlood Cell AggregationMicrovascular Blood FlowBlood Cell-endothelial Cell InteractionsCultured Endothelial CellsMicrocirculationBiophysical Pathophysiology
