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Hematologic रोग में Microvascular सहभागिता का अध्ययन करने के लिए endothelialized Microfluidics
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1Department of Pediatrics,Emory University School of Medicine, 2Wallace H. Coulter Department of Biomedical Engineering,Georgia Institute of Technology and Emory University, 3Aflac Cancer Center and Blood Disorders Service of Children’s Healthcare of Atlanta , 4Winship Cancer Institute of Emory University
Capitoli
- 00:05Titolo
- 01:11Fabrication of the Endothelial Microdevice
- 03:46Polydimethylsiloxane (PDMS) Preparation
- 06:25Seeding the Microfluidic Device with Endothelial Cells
- 08:49Endothelialized Microfluidics Reveal Abnormal Microvascular Interactions after Infusion with Blood from Patients with Hematologic Disease
- 10:35Conclusion
पूरे में microvascular आकार चैनल (<30 माइक्रोन) के साथ एक microfluidic डिवाइस के भीतर 3D सतह भर में एक endothelial सेल monolayer संस्कृति विधि वर्णित है. यह<em> इन विट्रो में</em> Microvasculature मॉडल रक्त कोशिकाओं, endothelial कोशिकाओं और hematologic रोगों में घुलनशील कारकों के बीच biophysical बातचीत के अध्ययन के लिए सक्षम बनाता है.
Tags
MicrofluidicsEndothelialized MicrofluidicsMicrovascular InteractionsHematologic DiseasesMicrofabrication TechniquesBiological ExperimentsBiochemical ExperimentsMicro- And NanoscalesHematologic ProcessesBlood Cell DeformabilityBlood Cell AggregationMicrovascular Blood FlowBlood Cell-endothelial Cell InteractionsCultured Endothelial CellsMicrocirculationBiophysical Pathophysiology
