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Isolering og kultur Rat Embryonale nevrale celler: En rask protokoll
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Neuroscienze
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JoVE Journal Neuroscienze
Isolation and Culture of Rat Embryonic Neural Cells: A Quick Protocol

Isolering og kultur Rat Embryonale nevrale celler: En rask protokoll

DOI:
10.3791/3965-v

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10:51 min

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May 24, 2012

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,
1LSU Health Sciences Center – New Orleans, 2Medical School and Stanley S. Scott Cancer Center

Capitoli

  • 00:05Titolo
  • 01:42Poly-D-Lysine: Preparation and Coating of the Plastic Cell Culture Dishes
  • 03:03PDL and Laminin: Preparation and Coating of the Glass Two-chamber Slides
  • 04:07Neuronal Dissection and Culture
  • 09:40Results: Isolation and Culture of Rat Embryonic Neural Cells
  • 10:27Conclusion

Summary

Traduzione automatica

Traduzione automatica

Vi beskriver en rask metode for å isolere og kultur hippocampus og kortikale nevroner fra gnagere embryoer. Denne protokollen tillater oss å utføre eksperimenter der nesten rene nevrale kulturer er nødvendig.

Tags

IsolationCultureRat Embryonic Neural CellsQuick ProtocolDissociationHippocampal NeuronsCortical NeuronsE15-17 Rat EmbryosMouse Primary NeuronsHuman Primary NeuronsNeural ProgenitorsSerum-free MediumNucleofectionImmunocytochemistryNucleic Acids PreparationElectrophysiologyLentiviral TransductionCalcium Phosphate TransfectionLipid-based Transfection

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