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实时数字成像大鼠提睾肌缺血再灌注损伤（IRI）的白细胞 - 内皮细胞相互作用
JoVE Journal
Medicina
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JoVE Journal Medicina
Real-time Digital Imaging of Leukocyte-endothelial Interaction in Ischemia-reperfusion Injury (IRI) of the Rat Cremaster Muscle
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实时数字成像大鼠提睾肌缺血再灌注损伤（IRI）的白细胞 - 内皮细胞相互作用

DOI:
10.3791/3973-v

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15:07 min

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August 05, 2012

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, , ,
1Department of Plastic and Hand Surgery,University of Freiburg Medical Centre

Capitoli

  • 00:05Titolo
  • 02:04Anesthesia and Monitoring
  • 08:00Preparation of the Cremaster Muscle
  • 11:27Intravital Setup
  • 13:15Offline Video Playback Analysis
  • 13:59Conclusion

Summary

Traduzione automatica

Traduzione automatica

数字提睾微循环毛细血管静脉活体荧光显微镜是一种方便的方法获得白细胞 - 内皮细胞相互作用的见解<em>在体内</em在缺血再灌注损伤（IRI）的横纹肌组织。我们在这里提供一份详细的协议，安全地执行技术，并讨论了它的应用和限制。

Tags

Real-timeDigital ImagingLeukocyte-endothelial InteractionIschemia-reperfusion InjuryIRIRat Cremaster MusclePathological ConditionsCerebral StrokeMyocardial InfarctionIntestinal IschemiaTransplantCardiovascular SurgeryReactive Oxygen SpeciesComplement SystemMicrovascular PermeabilityLeukocyte ActivationAdhesion MoleculesChemoattractantsSelectinsCounter LigandsIntercellular Adhesion MoleculesVascular Cellular Adhesion MoleculesIntravital Microscopy

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