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定向分化诱导性多潜能干细胞向T淋巴细胞
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Directed Differentiation of Induced Pluripotent Stem Cells towards T Lymphocytes
DOI:

12:47 min

May 14, 2012

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Capitoli

  • 00:05Titolo
  • 01:10Cell Culture and In vitro Programming
  • 02:15In vivo Maturation of Partially Differentiated iPS Cells
  • 03:36In vivo Programming
  • 04:51Adoptive Transfer and Peptide Stimulation
  • 05:50In vivo Killing Assay
  • 06:47Intraperitoneal Tumor Cell Count and Infiltrating Tumor Cell Identification
  • 08:43Results: In vivo Differentiation of TCR Gene-transduced iPS Cells into Functional Ag-specific CTLs
  • 12:25Conclusion

Summary

Traduzione automatica

诱导多能干细胞(iPS)细胞,T淋巴细胞的生成,给出了一个使用基于T细胞免疫治疗的胚胎干细胞的替代方法。该方法表明,利用任<em>在体外</em>或<em>在体内</em>感应系统,iPS细胞能够分化成传统和抗原特异性T淋巴细胞。

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