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Capitoli
- 00:05Titolo
- 01:33Imaging Plate Preparation
- 02:29Imaging Parameters for the Zeiss LSM 710 Confocal Microscope
- 03:37Optimizing the Imaging Parameters
- 04:49Mitochondrial-fusion Assay Set-up
- 07:36Analyzing the PA-GFP Signal Intensity
- 08:17Results: Representative Mitochondrial-fusion Assays
- 09:49Conclusion
Tags
FasterHigh ResolutionMtPA-GFP-based Mitochondrial Fusion AssayKinetic DataMultiple CellsParallelConfocal MicroscopyFusion ActivityPhoto-conversionPhotoactivatable GFP (mtPAGFP)EquilibrationLiving CellsTime Lapse ApproachScale UpAutomateLow Concentration Of TMRESignal IntensityPAGFP Expressing CellsSubcellular QuantificationCytoarchitectural Environment
