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Capitoli
- 00:05Titolo
- 02:03Sample Acquisition
- 04:24Development of Enrichment Cultures
- 06:42Cell Lysate Extraction from Filtered Enrichments
- 08:44RubisCO Carboxylase Activity Filter Assay
- 11:17Results: Analysis of Novel Protists from Lake Bonney, Antarctica
- 13:55Conclusion
Mikrobielle eukaryoter er både en kilde til photosynthetically-derived carbon og topp rov arter i permanent islagte Antarktis innsjøer. Denne rapporten beskriver en berikelse kultur tilnærming for å isolere metabolsk allsidige mikrobielle eukaryoter fra Antarktis innsjø, Lake Bonney, og vurderer uorganisk karbon fiksering potensial ved hjelp av en radioisotop analysen for ribulose-bifosfatase-karboksylase/oksygenase-proteinet-1 ,5-bisphophate carboxylase oxygenase (RubisCO) aktivitet.
Tags
Microbial EukaryotesAntarctic LakeChemically StratifiedCarbon Fixation PotentialLake BonneyIce-covered LakesMcMurdo Dry ValleysEnvironmental StressesNutrient DeficiencyLow TemperaturesExtreme ShadeHypersalinityDarknessMicroorganismsProtistsBiogeochemical CyclingCarbon CyclingAutotrophyPrimary ProducersTertiary RolesFood WebPhagotrophic ProtistsHeterotrophic ProtistsMixotrophic Metabolism
