Rivista
/
Neuroscienze
/
Retrograde Loading of Nerves, Tracts, and Spinal Roots with Fluorescent Dyes
JoVE Journal
Neuroscienze
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Neuroscienze
Retrograde Loading of Nerves, Tracts, and Spinal Roots with Fluorescent Dyes

Retrograde Loading of Nerves, Tracts, and Spinal Roots with Fluorescent Dyes

DOI:
10.3791/4008-v

•

06:47 min

•

April 19, 2012

•

,
1Developmental Neurobiology Section,National Institute of Neurological Disorders and Stroke, National Institutes of Health

Capitoli

  • 00:05Titolo
  • 01:44Pipette Preparation and Positioning
  • 02:40Dye Application
  • 04:36Incubation
  • 04:56Results: Retrograde Loading of Nerves, Tracts, and Spinal Roots with Fluorescent Dyes
  • 05:59Conclusion

Summary

Traduzione automatica

Traduzione automatica

We describe a simple and low cost technique for introducing high concentration of fluorescent and calcium-sensitive dyes into neurons or any neuronal tract using a polyethylene suction pipette.

Tags

Retrograde LabelingNervesTractsSpinal RootsFluorescent DyesCalcium-sensitive DyesVoltage-sensitive DyesSolid CrystalsLocal Pressure InjectionGlass PipettesDye DiffusionPolyethylene Suction Pipette

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/4008?article_type=v

Studying Synaptic Vesicle Pools using Photoconversion of Styryl Dyes
check_url/it/4008?article_type=v

Visualization of Larval Segmental Nerves in 3rd Instar Drosophila Larval Preparations
check_url/it/4008?article_type=v

Optical Imaging of Neurons in the Crab Stomatogastric Ganglion with Voltage-sensitive Dyes
check_url/it/4008?article_type=v

Retrograde Fluorescent Labeling Allows for Targeted Extracellular Single-unit Recording from Identified Neurons In vivo
check_url/it/4008?article_type=v

Promotion of Survival and Differentiation of Neural Stem Cells with Fibrin and Growth Factor Cocktails after Severe Spinal Cord Injury
check_url/it/4008?article_type=v

Retrograde Labeling of Retinal Ganglion Cells in Adult Zebrafish with Fluorescent Dyes
check_url/it/4008?article_type=v

In Vivo Electrophysiological Measurements on Mouse Sciatic Nerves
check_url/it/4008?article_type=v

A Procedure for Implanting a Spinal Chamber for Longitudinal In Vivo Imaging of the Mouse Spinal Cord
check_url/it/4008?article_type=v

Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors
check_url/it/4008?article_type=v

Retrograde Neuroanatomical Tracing of Phrenic Motor Neurons in Mice

Read Article