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定量健身分析工作流程
JoVE Journal
Biologia
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JoVE Journal Biologia
A Quantitative Fitness Analysis Workflow
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定量健身分析工作流程

DOI:
10.3791/4018-v

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11:39 min

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August 13, 2012

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, ,
1Institute for Cell and Molecular Biosciences,Newcastle University Medical School

Capitoli

  • 00:05Titolo
  • 02:39Manual Quantitative Fitness Analysis Protocol: Culturing Yeast Strains
  • 03:11Fully Automated Quantitative Fitness Analysis Protocol
  • 04:48Image and Experimental Metadata Capture: Automated Quantitative Fitness and Data Analysis
  • 09:32Representative Fitness Analysis Results
  • 10:40Conclusion

Summary

Traduzione automatica

Traduzione automatica

健身定量分析（QFA）是一系列估计微生物培养适应度的实验和计算方法的互补。 QFA估计的基因突变，药物或其他适用于微生物的生长治疗的效果。可以设计实验缩放从单一文化的集中分析数以千计的并行文化。

Tags

Quantitative Fitness AnalysisQFAFitness ComparisonMicrobial CulturesGenetic InteractionDrug ScreensGrowth CurvesQuantitative Cell Density EstimatesFitness MeasuresGenetic Interaction StrengthsDrug SensitivitiesSubstrate AgarSmall MoleculesAntibioticsNutrientsUV IrradiationTemperatureGrowth Rate EstimatesSpectrophotometric MeasurementHigh Throughput

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