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Preparing T Cell Growth Factor from Rat Splenocytes
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Biologia
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JoVE Journal Biologia
Preparing T Cell Growth Factor from Rat Splenocytes

Preparing T Cell Growth Factor from Rat Splenocytes

DOI:
10.3791/402-v

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18:27 min

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October 31, 2007

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,
1Department of Physiology and Biophysics,University of California, Irvine (UCI)

Capitoli

  • 00:00Titolo
  • 00:06Introduction
  • 01:13TCGF Secretion
  • 03:22Harvesting Spleens from Lewis Rats
  • 05:15Preparing Single Cell Suspensions from Rat Spleens
  • 10:23Lysis of Red Blood Cells
  • 12:40Preparing Splenocyte Culture
  • 13:34Elimination of the Conconavalin A
  • 17:53Conclusion

Summary

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We describe the preparation of T cell growth factor used for the in vitro expansion of antigen-specific rat T lymphocyte lines.

Tags

T Cell Growth FactorRat SplenocytesAntigen-specific T Cell LinesInterleukin 2Th1 PolarizationTCGF PreparationCytokinesLarge-scale ProductionRat Splenocyte Culture SupernatantsConcanavalin AMitogenCytokine ProductionSterile FiltrationStorage At -20°C

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