Rivista
/
Biologia
/
核糖体束缚新生多肽的分离<em>在体外</em>确定沿mRNA的翻译暂停网站
JoVE Journal
Biologia
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia
Isolation of Ribosome Bound Nascent Polypeptides in vitro to Identify Translational Pause Sites Along mRNA
Please note that all translations are automatically generated. Click here for the English version.

核糖体束缚新生多肽的分离<em>在体外</em>确定沿mRNA的翻译暂停网站

DOI:
10.3791/4026-v

•

10:15 min

•

July 06, 2012

•

,
1Center for Gene Regulation in Health and Disease, Department of Biological, Geological and Environmental Sciences,Cleveland State University

Capitoli

  • 00:05Titolo
  • 03:55DNA Template Preparation, In vitro Transcription, and In vitro Translation
  • 05:30Isolation and Resolution of Nascent Polypeptides
  • 06:54Representative Translational Pause Results
  • 08:35Conclusion

Summary

Traduzione automatica

Traduzione automatica

描述的一种技术，以确定mRNA的翻译暂停网站。本程序是基于对新生多肽在体外培养的靶mRNA的翻译，其次是使用变性凝胶电泳新生链的大小分析核糖体积累的隔离。

Tags

RibosomePolypeptidesTranslational Pause SitesMRNACodon UsageSynonymous CodonsNon-uniform ElongationMRNA Secondary StructureRibosome MovementTranslational Elongation RatesCodon BiasTRNAsRare CodonsPause SitesProtein ExpressionMRNA StabilityProtein Folding

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

PAR剪辑 - 一个方法来确定全转录组学，RNA结合蛋白的结合位点

在体外转录和Gaussia荧光素酶基因的上限由HeLa细胞转染

作为一种工具来隔离核糖体的束缚多肽SECM逮捕序列

在体外合成在人体细胞修饰蛋白表达的mRNA的感应

测量氧化应激性<em>秀丽隐杆线虫</em>在96孔微量滴定板

考试蛋白在哺乳动物细胞中绑定到新生的DNA使用的BrdU芯片插槽西方技术

测量的刚度<em>离体</em>鼠标主动脉用原子力显微镜

一个协议来使用基因集富集分析以确定合适的动物模型转化型研究

体外sumo 法研究相扑 E3 连接活动

从真核细胞 中快速体内 固定和分离转化复合物

Read Article