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Collection Protocol for Human Pancreas
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JoVE Journal Medicina
Collection Protocol for Human Pancreas

Collection Protocol for Human Pancreas

DOI:
10.3791/4039-v

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07:49 min

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May 23, 2012

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, , , , , ,
1Department of Pathology, Immunology, and Laboratory Medicine,University of Florida

Capitoli

  • 00:05Titolo
  • 01:26Preparation and Setup
  • 02:27Pancreas Dissection
  • 06:08Spleen, Lymph Node, and Duodenal Mucosa Dissection
  • 07:09Results: Pancreas Weights by Region
  • 07:25Conclusion

Summary

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This video demonstrates a dissection procedure for processing human pancreas into multiple storage formats. Anatomical orientation is maintained throughout the pancreatic regions to allow definition of regional islet composition and density.

Tags

Collection ProtocolHuman PancreasDissectionSampling ProcedureNetwork For Pancreatic Organ Donors With Diabetes (nPOD)StandardizeCadaveric Organ DonorsPancreas RegionsHeadBodyTailSerial Transverse SectionsFixed ParaffinFresh Frozen BlocksSnap Frozen Sample PreparationsRNAse InhibitorsDNARNAProtein IsolationPancreas Dissection ProcedureAnatomical OrientationEndocrine Cell HeterogeneityIslet CompositionSizeNumbersInsulin-containing β-cellsGlucagon-containing α-cellsSomatostatin-containing δ-cellsPancreatic Polypeptide-containing PP CellsGhrelin-containing Epsilon CellsUncinate RegionDevelopmental Differences

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