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补肾胶囊对糖尿病小鼠的胰岛移植
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Biologia
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JoVE Journal Biologia
Transplantation of Pancreatic Islets Into the Kidney Capsule of Diabetic Mice
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补肾胶囊对糖尿病小鼠的胰岛移植

DOI:
10.3791/404-v

•

10:43 min

•

October 31, 2007

•

, ,
1Diabetes Center,University of California, San Francisco – UCSF

Capitoli

  • 00:01Titolo
  • 00:17Introduction
  • 01:07Hand-Picking the Islets
  • 03:18Transferring Islets into P150 Tubing
  • 05:17Preparing the Mouse for Islet Transplant
  • 05:59Exposing the Kidney
  • 07:16Transplantation of Islets
  • 10:01Conclusion

Summary

Traduzione automatica

Traduzione automatica

我们的协议是干净，便捷地提供胰岛细胞或细胞小鼠肾囊下。集中使用，用于移植的肾囊下细胞最终油管颗粒细胞。这项技术的易用性，减少压力的细胞和鼠标。

Tags

TransplantationPancreatic IsletsKidney CapsuleDiabetic MiceProtocolCellsDelivery TubingPE50ConcentrationPelletsSpeedEaseStress ReductionLoadingMicrocentrifuge TubePipettemanPipette TipPE50 Tubing AttachmentSilicone Adapter TubingSettlingDialingKink15 ML ConicalCentrifugingAnesthesiaShavingCleaningIncisionKidney Exposure

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