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电脑断层导引的时域漫荧光体层摄影术在小动物的肿瘤生物标志物的本土化
JoVE Journal
Medicina
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JoVE Journal Medicina
Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers
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电脑断层导引的时域漫荧光体层摄影术在小动物的肿瘤生物标志物的本土化

DOI:
10.3791/4050-v

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12:24 min

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July 17, 2012

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, , , , , , , ,
1Thayer School of Engineering,Dartmouth College, 2Department of Physics and Astronomy,Dartmouth College, 3Darmouth Medical School,Dartmouth College, 4School of Computer Science,University of Birmingham

Capitoli

  • 00:05Titolo
  • 02:51Animal Preparation
  • 04:53Fluorescence Tomography System Calibration
  • 06:15Imaging Protocol
  • 08:45Image Reconstruction
  • 10:34Results: Fluorescence Reconstruction
  • 11:06Conclusion

Summary

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弥漫性荧光体层摄影术提供了一个相对较低的成本和潜在的高通量的方法，以临床<em>在体内</em>肿瘤显像。计算机断层扫描引导非接触式时域使用荧光针对肿瘤标志物的表皮生长因子受体在小鼠脑胶质瘤模型系统的光学数据收集，校准和图像重建方法。

Tags

Computed Tomography-guided Time-domain Diffuse Fluorescence TomographySmall AnimalsCancer BiomarkersPreclinical Drug DiscoveryDevelopment StudiesLight AbsorptionOptical Signal PropagationVisible WavelengthsRed And Near-infrared (near-IR) Light AbsorptionNon-elastic ScatteringFluorescent AgentsImaging SystemsLight Propagation ModelsWhole Body Three-dimensional ImagingIll-posed Nature Of Diffuse Fluorescence TomographyStabilityContrast RecoverySpatial ResolutionImage Reconstruction TechniquesFMI In Small AnimalsCharge-coupled Device (CCD)-based SystemsSensitivity Detectors

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