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Immunology and Infection

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JoVE Journal Immunology and Infection
Non-invasive Imaging of Disseminated Candidiasis in Zebrafish Larvae
 

Non-invasive Imaging of Disseminated Candidiasis in Zebrafish Larvae

Article DOI: 10.3791/4051-v • 10:45 min • July 30th, 2012
July 30th, 2012 •
1, 1
1Department of Molecular and Biomedical Sciences, University of Maine

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The rapid development, small size and transparency of zebrafish are tremendous advantages for the study of innate immune control of infection1-4. Here we demonstrate techniques for infecting zebrafish larvae using the fungal pathogen Candida albicans by microinjection, methodology recently used to implicate phagocyte NADPH oxidase activity in control of fungal dimorphism5.

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Non-invasive Imaging Disseminated Candidiasis Zebrafish Larvae Candida Albicans Innate Immunity Genetic Defects Phagocyte NADPH Oxidase Dynamics Of C. Albicans Interaction Macrophages Neutrophils In Vitro Studies In Vivo Environment Cytokine Levels Extracellular Matrix Attachments Intercellular Contacts Model Organism Live Intact Host Zebrafish Larva Innate Immune Defenses
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