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人类胰岛染色协议

JoVE Journal
Medicina

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JoVE Journal Medicina

Staining Protocols for Human Pancreatic Islets

Please note that all translations are automatically generated. Click here for the English version.

人类胰岛染色协议

DOI:

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07:48 min

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May 23, 2012

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Martha L. Campbell-Thompson, Tiffany Heiple, Emily Montgomery, Li Zhang, Lynda Schneider

¹Department of Pathology, Immunology, and Laboratory Medicine,University of Florida

Capitoli

00:05Titolo
01:12Microtomy for Unstained Sections
02:50H&E Staining
03:45Immunohistochemistry (IHC) Procedure
05:19Dehydration
06:16Slide Labeling and Scanning
07:05Results: Staining of Pancreatic Sections
07:32Conclusion

Summary

Traduzione automatica

本视频演示了人体胰腺胰岛细胞用苏木精伊红（H＆E）和免疫组化法（IHC）的鉴定程序。从头部，身体，和尾区域胰腺部分是染色都Ĥ＆é和免疫组化确定胰岛内分泌（胰岛素，胰高血糖素，和胰多肽）组成，细胞复制（Ki67的）和（H＆E，CD3）炎性浸润。钩突地区的本地化使用胰多肽免疫组化。

Tags

Staining Protocols Human Pancreatic Islets Islet Area Endocrine Cell Composition Processing And Staining Procedure Histopathology Immunolocalization Examinations Standardized Procedures Transverse Sections Pancreatic Head Pancreas Tail Serial Sectioning Histopathological Characterization Islet Endocrine Cells Replication T-cell Infiltrates Exocrine Compartment Ductular Components

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