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钙成像的急性下区片的制备

JoVE Journal
Neuroscienze

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JoVE Journal Neuroscienze

Preparation of Acute Subventricular Zone Slices for Calcium Imaging

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钙成像的急性下区片的制备

DOI:

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09:10 min

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September 19, 2012

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Benjamin Lacar, Stephanie Z. Young, Jean-Claude Platel, Angélique Bordey

¹Department of Neurosurgery and Cellular & Molecular Physiology,Yale University School of Medicine

Capitoli

00:05Titolo
01:02Removal of Brain Tissue
02:03Acute Brain Slice Preparation
03:33Preparation of the Microscope and Dyes for Imaging
04:50Dye Loading: The Bath Method
05:46Dye Loading: The Pressure Application Method
07:06Confocal Imaging of Calcium Activity
08:02Results: Calcium Activity in the SVZ
08:55Conclusion

Summary

Traduzione automatica

一个方法来加载脑室下区（SVZ）细胞中的钙离子指示剂染料记录活性钙。产后SVZ包括神经祖细胞和神经母细胞的细胞排列紧密。而不是使用清洗负载注入染料内的组织可以更好的染料扩散的压力。

Tags

Acute Subventricular Zone Slices Calcium Imaging Neurogenic Zone SVZ Astrocytes Neuroblasts Intermediate Progenitor Cells Interneurons Adhesion Molecules Diffusible Signals Intercellular Calcium Activity Functional Intercellular Signaling Calcium Indicator Dye Bath Loading Cellular Density Sagittal Slices Three-dimensional Arrangement Neuroblast Migration

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