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Capitoli
- 00:05Titolo
- 01:02Removal of Brain Tissue
- 02:03Acute Brain Slice Preparation
- 03:33Preparation of the Microscope and Dyes for Imaging
- 04:50Dye Loading: The Bath Method
- 05:46Dye Loading: The Pressure Application Method
- 07:06Confocal Imaging of Calcium Activity
- 08:02Results: Calcium Activity in the SVZ
- 08:55Conclusion
En metode for å laste subventricular sone (SVZ) celler med kalsium indikator fargestoffer for opptak kalsium aktivitet er beskrevet. Postnatal SVZ inneholder tett pakket celler, inkludert nevrale stamceller og neuroblasts. Snarere enn å bruke bad lasting injisert vi fargestoff ved trykk inne i vevet lar bedre fargestoff diffusjon.
Tags
Acute Subventricular ZoneSlicesCalcium ImagingNeurogenic ZoneSVZ AstrocytesNeuroblastsIntermediate Progenitor CellsInterneuronsAdhesion MoleculesDiffusible SignalsIntercellular Calcium ActivityFunctional Intercellular SignalingCalcium Indicator DyeBath LoadingCellular DensitySagittal SlicesThree-dimensional ArrangementNeuroblast Migration
