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基因转移到老年人的鸡胚<em>前OVO</em>电穿孔

JoVE Journal
Biologia

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JoVE Journal Biologia

Gene Transfer into Older Chicken Embryos by ex ovo Electroporation

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基因转移到老年人的鸡胚<em>前OVO</em>电穿孔

DOI:

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06:10 min

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July 27, 2012

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Jiankai Luo, Xin Yan, Juntang Lin, Arndt Rolfs

¹Albrecht-Kossel-Institute for Neuroregeneration,School of Medicine University of Rostock, ²Institute of Anatomy I,School of Medicine University of Jena

Capitoli

00:05Titolo
01:22Ex ovo Culture
02:51Preparation for Ex ovo Electroporation
03:51Gene Transfer into Chicken Optic Tectum by Ex ovo Electroporation
04:43Results: Overexpression of Cad7 and GFP by Ex ovo Electroporation
05:50Conclusion

Summary

Traduzione automatica

介绍将在以后的孵化阶段（比汉堡和汉密尔顿阶段（HH）22岁以上）的鸡胚胎的基因转移方法。这种方法克服缺点<em>在OVO</em>电适用于年龄较大的鸡胚胎是一种有用的技术，研究基因功能和调控，在旧的发展阶段。

Tags

Gene Transfer Chicken Embryos Ex Ovo Electroporation Gene Function Gene Regulation In Vivo DNA Plasmids SiRNA Plasmids RNA Oligos Morpholino Antisense Oligonucleotides Incubation Stages Vitelline Membrane Allantoic Vessels Gene Transfer Protocol

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