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Assessing Replication and Beta Cell Function in Adenovirally-transduced Isolated Rodent Islets
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Assessing Replication and Beta Cell Function in Adenovirally-transduced Isolated Rodent Islets

Assessing Replication and Beta Cell Function in Adenovirally-transduced Isolated Rodent Islets

DOI:
10.3791/4080-v

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09:31 min

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June 25, 2012

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, , ,
1Department of Pediatrics,Indiana University School of Medicine, 2Department of Cellular & Integrative Physiology,Indiana University School of Medicine

Capitoli

  • 00:05Titolo
  • 00:58Adenoviral Transduction and Culturing of Rat Islets
  • 03:26Insulin Secretion Assay
  • 06:13Thymidine Incorporation Assay
  • 07:49Beta Cells Overexpressing “Gene #6”
  • 09:09Conclusion

Summary

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This protocol allows one to identify factors that modulate functional beta cell mass to find potential therapeutic targets for the treatment of diabetes. The protocol consists of a streamlined method to assess islet replication and beta cell function in isolated rat islets following manipulation of gene expression with adenoviruses.

Tags

ReplicationBeta Cell FunctionAdenovirally-transduced Isolated Rodent IsletsGlucose HomeostasisInsulinGlucagonPancreatic Beta CellsAlpha CellsFunctional Beta Cell MassNutrient LoadDiabetesAutoimmune AttackType 1 DiabetesType 2 DiabetesInsulin SecretionMolecular PathwaysTherapeutic TargetsBeta Cell GrowthImpairing Beta Cell FunctionOncogenesTarget Genes

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