Rivista
/
Medicina
/
复制和β细胞功能评估Adenovirally转分离的啮齿动物胰岛
JoVE Journal
Medicina
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Medicina
Assessing Replication and Beta Cell Function in Adenovirally-transduced Isolated Rodent Islets
Please note that all translations are automatically generated. Click here for the English version.

复制和β细胞功能评估Adenovirally转分离的啮齿动物胰岛

DOI:
10.3791/4080-v

•

09:31 min

•

June 25, 2012

•

, , ,
1Department of Pediatrics,Indiana University School of Medicine, 2Department of Cellular & Integrative Physiology,Indiana University School of Medicine

Capitoli

  • 00:05Titolo
  • 00:58Adenoviral Transduction and Culturing of Rat Islets
  • 03:26Insulin Secretion Assay
  • 06:13Thymidine Incorporation Assay
  • 07:49Beta Cells Overexpressing “Gene #6”
  • 09:09Conclusion

Summary

Traduzione automatica

Traduzione automatica

该协议允许一个确定调节功能的β细胞质量的因素，找到治疗糖尿病的潜在的治疗靶点。该协议包括一个精简的方法来评估胰岛细胞复制和β细胞功能，在离体大鼠胰岛细胞与腺病毒基因表达操纵。

Tags

ReplicationBeta Cell FunctionAdenovirally-transduced Isolated Rodent IsletsGlucose HomeostasisInsulinGlucagonPancreatic Beta CellsAlpha CellsFunctional Beta Cell MassNutrient LoadDiabetesAutoimmune AttackType 1 DiabetesType 2 DiabetesInsulin SecretionMolecular PathwaysTherapeutic TargetsBeta Cell GrowthImpairing Beta Cell FunctionOncogenesTarget Genes

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/4080?article_type=v

在分离的小鼠使用红桃心功能和能量学的评估 31 P核磁共振
check_url/it/4080?article_type=v

评估与远藤的PAT 2000内皮血管舒张功能
check_url/it/4080?article_type=v

离体骨骼肌肌的力学分析
check_url/it/4080?article_type=v

从部分Pancreatectomized患者的人类胰岛的分离
check_url/it/4080?article_type=v

在斑马鱼模型的胎儿酒精暴露评估致畸的变化
check_url/it/4080?article_type=v

人类胰岛染色协议
check_url/it/4080?article_type=v

瘦素受体<sup>分贝</sup> 2型糖尿病小鼠模型：胰岛分离和活细胞双光子成像完好胰岛
check_url/it/4080?article_type=v

高含量<em>体外</em>胰岛β细胞复制发现平台
check_url/it/4080?article_type=v

定量3D<em>在硅片</em>建模阿尔茨海默病的啮齿动物模型脑淀粉样蛋白-β吞噬（q3DISM）
check_url/it/4080?article_type=v

在女小鼠害保留神经的中期尿道梗阻（NEMO）

Read Article