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突触荧光自动定量<em> C。线虫</em

JoVE Journal
Neuroscienze

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JoVE Journal Neuroscienze

Automated Quantification of Synaptic Fluorescence in C. elegans

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突触荧光自动定量<em> C。线虫</em

DOI:

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12:22 min

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August 10, 2012

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Brianne L. Sturt, Bruce A. Bamber

¹Department of Biological Sciences,University of Toledo

Capitoli

00:05Titolo
01:29Preparation of Worms for Imaging
04:50Confocal Imaging
07:34Automated Identification and Analysis of Individual Synaptic Clusters
10:28Representative Results
11:58Conclusion

Summary

Traduzione automatica

强烈的大量聚集在突触的神经递质受体影响突触强度。这种方法量化单突触决议的荧光标记的神经递质受体在三个层面<em> C。线虫</em>，让数百个突触被迅速的特点是在一个单一的样本没有推出Z-平面上的投影的扭曲。

Tags

Automated Quantification Synaptic Fluorescence C. Elegans Synapse Strength Neurotransmitter Receptors Synaptic Sites Postsynaptic Membrane Receptor Trafficking Synaptic Plasticity Neuromodulation Fluorescence Microscopy Synaptically-localized Neurotransmitter Receptor Abundance Synaptic Development Synaptic Plasticity Caenorhabditis Elegans Native Synapses Automated Identification Three Dimensions

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