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在小鼠体内的巨噬细胞的枯竭和重建
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Depletion and Reconstitution of Macrophages in Mice
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在小鼠体内的巨噬细胞的枯竭和重建

DOI:
10.3791/4105-v

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08:50 min

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August 01, 2012

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, ,
1Department of Graduate Studies,University of British Columbia , 2Department of Molecular Biology,Vrije Universiteit Amsterdam, 3Department of Pediatrics,University of British Columbia

Capitoli

  • 00:05Titolo
  • 01:12Depleting Macrophages using Clodronate-containing Liposomes
  • 02:15Derivation of Polarized Macrophages from Bone Marrow Aspirates
  • 04:24Transferring Macrophages into Mice
  • 05:17Results: Clodronate Liposome Depletion of Macrophages Allows Adoptive Transfer of Experimental Macrophage Populations
  • 08:23Conclusion

Summary

Traduzione automatica

Traduzione automatica

巨噬细胞发挥核心作用的动态平衡，并在许多组织的病理。这里介绍的协议描述消耗巨噬细胞的方法<em>在体内</em>，来自偏光巨噬细胞，骨髓穿刺，继转移到小鼠巨噬细胞。这些技术允许极化的巨噬细胞在健康和疾病中发挥的作用的决心。

Tags

DepletionReconstitutionMacrophagesMiceInnate Immune ResponseAcquired Immune ResponseMacrophage DysfunctionDisease ProcessesInflammatory Bowel DiseasesPolarized PhenotypesClassically Activated MacrophagesAlternatively Activated MacrophagesIFN-gammaLPSIL-4IL-13InflammationInflammatory ResponseWhole Animal ModelDepleting MacrophagesComplex EnvironmentPhenotypically Defined Polarized MacrophagesBone Marrow Aspirates

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